Glucose Oxidase 抗体 (HRP)
Quick Overview for Glucose Oxidase 抗体 (HRP) (ABIN5596887)
抗原
适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 200-6337-0100
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Supplier
- Rockland
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原理
- Glucose Oxidase Antibody Peroxidase Conjugated
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Sheep Serum as well as purified and partially purified Glucose Oxidase [Aspergillus niger].
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产品特性
- Synonyms: sheep anti-Glucose Oxidase HRP conjugated Antibody, peroxidase conjugated sheep anti-Glucose Oxidase Antibody, Beta D Glucose Oxygen 1 Oxido Reductase antibody, Glucose oxidase (Precursor) antibody, Glucose Oxyhydrase antibody, GOD antibody
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纯化方法
- Anti-Glucose Oxidase (Sheep) antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
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免疫原
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Immunogen: Glucose Oxidase [Aspergillus niger]
Immunogen Type: Native Protein
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亚型
- IgG
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anti-Glucose Oxidase (GOD) antibody
GOD 适用: Aspergillus niger ELISA, WB, IHC 宿主: 山羊 Polyclonal unconjugated
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应用备注
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Application Note: Anti-Glucose Oxidase peroxidase conjugated antibody has been tested by dot blot and is suitable to be assayed against 1.0 μg of Glucose Oxidase [Aspergillus niger] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:3,000 of the reconstitution concentration is suggested for this product.
Western Blot Dilution: User Optimized
ELISA Dilution: User Optimized
Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
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浓度
- 1 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! -
储存液
- Gentamicin sulfate
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注意事项
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- Glucose Oxidase (GOD)
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别名
- Glucose Oxidase
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背景
- Background: Glucose oxidase is an enzyme that drives the oxidation of glucose into hydrogen peroxide and D-glucono-δ-lactone. This activity is utilized in biotechnology through colormetric detection assays that are sensitive to the amount of hydrogen peroxide produced. Horseradish Peroxidase (HRP) is an enzyme that utilizes organic peroxide compounds as electron donors. Horseradish Peroxidase naturally provides protection for plants against pathogens, but can be utilized in molecular biology to convert various substrates to detectable compounds (such as in Western Blotting and ELISAs). Anti-Glucose Oxidase (Sheep) antibody is ideal for investigators in Microbiology, Immunology, and Cell Biology research.
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UniProt
- P13006
抗原
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